Hitrap hp pdf complete

Hitrap protein a hp, 1 ml and 5 ml hitrap protein a hp is a prepacked re ady to use, column for preparative purification of monoclonal and polyclonal antibodies. Ge healthcare, little chalfont, uk and eluted using a nacl step gradient. The two media remain charged and have high loading capacities over broad ph. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. Q sepharose high performance is a strong anion exchange medium and sp sepharose high performance a strong cation exchanger. The special design of the column, together with the medium, provide fast, simple and easy separations in a convenient format.

Hitrap rprotein a ff, hitrap protein a hp, hitrap protein g hp. Combine chromatography techniques to optimize your protein. Histrap high performance ge healthcare, 29051021, pack of. The reuse of hitrap chelating hp depends on the nature of the sample and should only be. Free hp pdf complete download hp pdf complete driver top 4 download offers free software downloads for windows, mac, ios and android computers and mobile devices. The ionic strength must be kept as low as possible to allow all components to bind to the ionic exchange complete picture of the whole sample.

Hewlettpackard relied on pdf complete to provide an amazing outofbox experience for their customers. Instructions 71715400 ak hitrap desalting columns ge healthcare hitrap desalting hitraptm desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i. Histrap hp columns are designed for simple, onestep purification of histidinetagged proteins. Itih4 fractions, eluted between 450 mm and 1 m nacl, were desalted and separated via reversedphase chromatography on a proswift rp1s column 4. Histrap hp columns from ge healthcare biocompare product. Hitrap desalting hiprep 2610 desalting introduction hitrap desalting is a prepacked, ready to use 5 ml column for fast and convenient group separations between high and low molecular weight substances fig 1. Column hitrap q hp and hitrap sp hp are 1 ml and 5 ml columns made of polypropylene, which is biocompatible and noninteractive with biomolecules. Hiprep 2610 desalting is a prepacked ready to use 53ml column for larger sample volumes fig 2. If you have already purchased a product from our online store, click below and login to your account to view your available downloads. Hitrap q hp or hitrap sp hp, hitrap capto q impres, or hitrap capto sp impres sec final removal of salt and remaining impurities superdex 75 increase1, hiload superdex 75 pg, hiprep sephacryl s100 hr, hiprep sephacryl s200 hr sec final removal of remaining impurities superdex 75 increase, hiload superdex 75 pg. You can view this document in free acrobat reader, navigate through the page or the whole document which is one or more pages usually.

Hitrap q hp are prepacked, readytouse q sepharose high performance strong anion exchange columns for highresolution, smallscale protein purification. The q functional group is a quaternary amino group and the sp sulphopropyl. These advanced elements include a streamlined user interface, improved editing, and better than ever microsoft office conversions. Hitrap protein a hp, 1 ml ge healthcare superdex 200, 10300 gl, m, 10. Hitrap heparin hp 12 index of references3 ref 18115675 edition ab, 200204 53. Combines the basic tools of corporate edition plus your most requested office edition features. Pdf complete corporate edition software wq534at pdf complete corporate edition software wq534at product documentation.

The columns are prepacked with ni sepharose high performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target. The columns can be operated with a syringe, peristaltic pump or liquid. Sepharose high performance, has a small bead size 34 m. Recommended flow rates for tubing, uvvis flow cells, and. We are proud to be part of delivering pdf solutions to hewlettpackard customers. Performing a separation with hitrap igm purifcation hp. Hitrap protein a hp bioscale mini unosphere supra 1 and 5 7324200 and 7324202 gstrap hp bioscale mini profinity gst 1 and 5 pkg of 5. Ge healthcare life sciences product part list official.

Cell debris was removed by centrifugation and filtered at 4 c through a sterile 0. The drawback of this strategy is that the binding capacity. In my experiments, when i used a 5 ml column to purify 2 to 5 mg vegfhis from about ml of yeast expression medium, onethird of my protein did not bind to the column in the first loading. The ionic strength should be kept as low as possible to allow all components to bind to the ionic exchange hp pdf complete driver top 4 download offers free software downloads for windows, mac, ios and android computers and mobile devices.

From 2003 to 2016, hewlettpackard desktop, notebook, tablet, and workstation computers included pdf complete software. Purifications were also run on 1 ml hitrap columns with mabselect sure. Hitrap streptavidin hp column are convenient and reliable prepacked columns. Optimization of a calciumdependent protein aderived domain. Pdf complete end user license agreement pdf complete end user license agreement. Convenient and reproducible for fast, easy, highperformance separations either alone or connected in series. Instructions 71700500 ax hitrap affinity columns ge healthcare hitrap chelating hp, 1 ml and 5 ml hitrap chelating hp is a prepacked ready to use, column for preparative affinity chromatography. Both are coupled to the matrix via chemically stable ether linkages. Combining smallscale purification and analysis of monoclonal. This software demonstrates the functionality of pdf complete corporate edition and will place a demonstration watermark on each page. Histrap hp 1 ml and 5 ml hebrew university of jerusalem. The special design of the column, together with the high performance. The neutralized material was loaded onto two 1ml hitrap q ff ge healthcare columns in sequence.

Ge healthcare hitrap streptavidin hp column 5 x 1ml. The columns are prepacked with ni sepharose high performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated imac purifications. Hitrap q hp and hitrap q ff 12 index of references3 ref 18115682 edition ab, 200204 51. Pdf q sepharose high performance and sp sepharose high. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format. Hitrap q hp from ge healthcare life sciences biocompare. Glutathione sepharose 4 fast flow bead size 90 m with glutathione sepharose high performance in a purification of gsthis m r 26 000, expressed in e. The glutathione sepharose high performance peaks are both narrower and sharper which indicates a more concentrated sample.

The top and bottom frits are manufactured from porous polyethylene. After the protein has eluted, regenerate the column by washing it with start buffer according to step 3. Jul 24, 2006 the histrap hp column capacity is reported to be at least 40 mg of 6histidinetagged proteinml medium. Hitrap rprotein a ff, hitrap protein a hp and hitrap protein g hp are part of the range of prepacked, ready to use columns for preparative a. Data file 18117288 ab ion exhange chromatography q. The z ca variants coupled to a solid support and packed in hitrap columns, as well as the monomeric z ca, without a cterminal cysteine, as developed in kanje et al. The drawback of this strategy is that the binding capacity of the ion. Pdf file format, developed by adobe systems, represents in electronic mode all the elements of a printed document such as text, photos, links, scales, graphs and interactive elements. Hitrap chelating hp, 1 ml and 5 ml hebrew university of.

Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml. Hitrap affinity columns histrap hp, 1 ml and 5 ml histrap hp is a prepacked, readytouse column for the preparative purification of histagged recombinant proteins by immobilized metal affinity chromatography imac using precharged ni sepharose high performance. Hitrap protein g hp columns are prepacked with protein g sepharose high performance for fast, easy separations of igg from different species and subclasses. The concentrated supernatants were then passed over five 5ml protein g hitrap hp columns ge healthcare in sequence, and the bound igg was eluted with 50 mm sodium bicarbonate, ph 11. Hitrap blue hp, 1 ml and 5 ml hitrap blue hp is a prepacked ready to use, column for preparative affinity chromatography. The ionic strength should be kept as low as possible to allow all components to bind to the ionic exchange complete picture of the whole sample.

Fast, simple and easy separations are provided by the combination of easy to use hitrap column and the various a. Free hp pdf complete download hp pdf complete driver top. Jan 02, 20 my hp p6710f came with pdf complete which lets you save a single page of a pdf as its own pdf file. The column is now ready for a new purification and there is no need to reload with metal. High binding capacity of 25 mg iggml medium and fast binding kinetics. The interaction between the protein and the ligand has been suggested to result from the combined electron donating and acceptingaction of the ligand in a mixed mode hydrophilichydrophobic interaction. Characteristics of hitrap sp hp and hitrap q hp, 1 and 5 ml columns are listed in table 1.